Low percentage of aluminoxamine and ferroxamine in uremic serum after desferrioxamine administration

Abstract

HPLC was used to study the effectiveness of two different desferrioxamine (DFO) administration strategies (15 mg/kg DFO, 1 h or 44 h before dialysis) on generation of aluminoxamine and ferrioxamine in five hemodialysis patients. The percentage of ultrafilterable aluminum and iron in these patients was also investigated by electrothermal atomic absorption spectrometry. The administration of DFO in both schemes increased the ultrafilterable serum aluminum concentrations from a mean of 17.1 ± 1.6% to a mean of 75.7 ± 14.1%. However, 1 h after DFO infusion, only 38.8 ± 7.7% of the total serum aluminum was bound to DFO; 44 h after DFO infusion, only 15.8 ± 8.0% was bound. Similar results were obtained for ferrioxamine. These results suggest that the ultrafilterable serum faction contains aluminum and iron chelated by DFO and by DFO metabolites, which retain similar metal- chelating abilities.