Cloning of the ILV2, ILV3 and ILV5 genes of Saccharomyces cerevisiae

Abstract

The molecular analysis of the ILV genes which code for enzymes catalyzing steps in the biosynthesis of isoleucine and valine, is a prerequisite for the eincidation of the pathway-specific and general amito acid control mechanisms regulating isoleucine and valine biosynthesis in Saccharomyces. The ILV2, ILV3 and ILV5 genes of Saccharomyces cerevisiae have been cloned by complementation of mutations in these genes. Southern hybridization analysis shows that the cloned genes represent unique sequences of the wild-type genome of S. cerevisiae. The identity of the cloned genes is demonstrated by their specificity in the complementation of a collection of mutant alleles of the ILV genes and by site-directed integration of the cloned fragments at the ILV loci. © 1984 Carlsberg Laboratory.