Chicken Toes-Like Leaf and Petalody Flower (CTP) is a novel regulator that controls leaf and flower development in soybean

Abstract

A soybean mutant displaying chicken toes-like leaves and petalody flowers was identifed as being caused by a single recessive gene, termed ctp. Using heterozygous-inbred recombinants, this gene was fne-mapped to a 37-kb region harbouring three predicted genes on chromosome 5. The gene Glyma05g022400.1 was detected to have a 33-bp deletion in its frst exon that was responsible for ctp. Validation for this gene was provided by the fact that the 33-bp deletion-derived marker I2 completely co-segregated with the phenotypes of 96 F10-derived residual heterozygous lines and 2200 fne-mapping individuals, and by the fact that the orthologue NbCTP in Nicotiana benthamiana also in?uenced leaf and flower development under virus-induced gene silencing. In terms of characterization, the CTPs shared highly conserved domains specifcally in higher plants, GmCTP was alternatively spliced, and it was expressed in multiple organs, especially in lateral meristems. GmCTP was localized to the nucleus and other regions and performed transcriptional activity. In ctp, the expression levels and splicing patterns were dramatically disrupted, and many key regulators in leaf and/or floral developmental pathways were interrupted. Thus, CTP is a novel and critical pleiotropic regulator of leaf and flower development that participates in multiple regulation pathways, and may play key roles in lateral organ differentiation as a putative novel transcription regulator.