Exploring Brain Genoarchitecture by single and double chromogenic in situ hybridization (ISH) and immunohistochemistry (IHC) in whole-mount embryos

Abstract

This chapter and 5 detail various in situ hybridization protocols perfected over time in two laboratories with widespread experience in studying brain development and comparative neuroanatomy in amphibian, avian, and mammalian animal models. The aims of our research have frequently revolved on studying highquality maps of gene expression-genoarchitecture-in the developing or adult brain. Apart of the protocols themselves, in this chapter, detailed advice is given as well about ways to treat the brain specimens to insure little damage and good histological quality during whole-mount ISH and IHC.