Avaliação de reagentes anti-D na detecção dos antígenos D fraco e D parcial

0Citations
Citations of this article
27Readers
Mendeley users who have this article in their library.

Abstract

Monoclonal antibodies (MoAb) anti-D IgG and IgM are being developed in order to replace polyclonal antibodies. However, there are few studies about the selection of these MoAb to routinely detect weak D and partial D antigens. A total of 56 weak D blood samples were analyzed with anti-D IgG and IgM MoAb in order to evaluate their reactivity. Molecular analyses were also performed to characterize the weak D types and the presence of partial D. Antigen densities were determined by flow cytometry. Weak D type 1, 3 and 4 samples with high antigen density were reactive with the MoAb anti-D IgM at room temperature while weak D type 2 and the partial D samples with low antigen density were detected with MoAb anti-D IgG using the indirect antiglobulin test. Our results show that anti-D IgM does not detect partial D samples reactive as weak D and thus can be used for routine testing of patients. As the anti-D IgG detected all the weak D and partial D with low antigen density, it should be used in the routine testing of donors.

Author supplied keywords

Cite

CITATION STYLE

APA

Barros, C., Otta, M., Wakim, V. L., Zaqueroni, M., Baleotti, W., & Castilho, L. (2007). Avaliação de reagentes anti-D na detecção dos antígenos D fraco e D parcial. Revista Brasileira de Hematologia e Hemoterapia, 28(4), 269–274. https://doi.org/10.1590/s1516-84842006000400010

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free