We describe the utility of intermediate-pressure MALDI and tandem mass spectrometry (MS/MS and MS n) for the characterization and imaging of phospholipids in brain tissue sections. The use of both MS/MS spectra and MS/MS images allows for identification of isobaric compounds. The structural characterization of phosphatidylcholines, phosphatidylserines, phosphatidylethanolamines, and sphingomyelins directly from tissue sections is described. © 2010 Springer Science+Business Media, LLC.
CITATION STYLE
Garrett, T. J., & Yost, R. A. (2010). Tandem mass spectrometric methods for phospholipid analysis from brain tissue. Methods in Molecular Biology, 656, 209–230. https://doi.org/10.1007/978-1-60761-746-4_12
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