Purification of AJLec: A novel galactose-specific lectin from the sea anemone anthopleura japonica

Abstract

AJLec (18.5 kDa), a novel galactose-specific lectin, was isolated from the sea anemone Anthopleura japonica. AJLec demonstrates specificity for galactose monomers and β-linked terminal galactose residues in complex carbohydrates but not for N-acetylgalactosamine (GalNAc) which is commonly recognized by the galactose-binding lectins. Here, we have described the characteristics and process of extraction and purification of AJLec from Anthopleura japonica, which is useful for the analysis of complex carbohydrates.