Pectic polysaccharides from dietary sources such as Decalepis hamiltonii-swallow root (SRPP), Hemidesmus indicus (HPP), Nigella sativa-black cumin (BCPP), Andrographis serpyllifolia-(APP), Zingiber officinale-ginger (GRPP) and, citrus pectin (CPP) were examined for galectin inhibitory activity. Inhibition of (a) galectin-3 of MDA-MB-231 cells induced hemagglutination of red blood cells; (b) galectin-3 mediated interaction between normal/metastatic human buccal cells (NBC)/(MBC) and; (c) invasion of MDA-MB-231 and MBC in the invasive chamber was assessed. Results indicated that SRPP inhibited hemagglutination at Minimum Inhibitory Concentration (MIC) of 1.86 μg ml -1 equivalent of carbohydrate as apposed to those of BCPP (130 μg ml-1), APP (40 μg ml-1), HPP (40 μg ml-1) and CPP (25 μg ml-1). GRPP even at concentration >1-6 mg ml-1 did not inhibit agglutination. Also SRPP showed ∼15 and 2 fold potent anti hemagglutination activity relative to that of galectin-3 specific sugars-galactose (MIC-27.1 μg ml-1) and lactose (MIC-4.16 μg ml-1) respectively. Further, SRPP at 10 μg ml-1 inhibited agglutination of NBC by galectin-3 of MDA-MB-231 cells. Modified swallow root pectic polysaccharide (MSRPP) of 50 kDa retained anti hemagglutination activity (MIC of 1.03 μg ml-1) and inhibited MDA-MB-231 and MBC invasion by 73 and 50% with an IC50 of 136 and 200 μg ml-1 respectively. Both SRPP and MSRPP induced apoptosis up to 80% at 100 μg ml-1 concentration by activating ∼2 and 8 folds of Caspase-3 activity. Sugar composition analysis and its correlation with the galectin inhibitory property indicated that pectic polysaccharides with higher arabinose and galactose content-arabinogalactan inhibited hemagglutination significantly. © 2007 Springer Science+Business Media, LLC.
CITATION STYLE
Sathisha, U. V., Jayaram, S., Harish Nayaka, M. A., & Dharmesh, S. M. (2007). Inhibition of galectin-3 mediated cellular interactions by pectic polysaccharides from dietary sources. Glycoconjugate Journal, 24(8), 497–507. https://doi.org/10.1007/s10719-007-9042-3
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