1-Aminocyclopropane-1-carboxylic acid (ACC) is a biosynthetic precursor of ethylene. The movement of ACC across the plasma membrane (PM) has been implicated in various physiological contexts during environmental adaptation and differentiation in higher plants. A PM-localized transporter in Arabidopsis thaliana, LYSINE HISTIDINE TRANSPORTER1 (LHT1) participates in the uptake of ACC, implicating a class of amino-acid transporters in the transport of ACC. Here, we describe the method for assaying uptake of ACC into the plant cells using an Arabidopsis mesophyll protoplast system.
CITATION STYLE
Song, W. Y., Lee, S., & Soh, M. S. (2017). Analysis of 1-aminocyclopropane-1-carboxylic acid uptake using a protoplast system. In Methods in Molecular Biology (Vol. 1573, pp. 41–46). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6854-1_4
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