Development and validation of a method for simultaneous estimation of metformin and sitagliptin in human plasma by LC-MS-MS and its application in a bioequivalence study

46Citations
Citations of this article
42Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

A simple, sensitive, precise and accurate method for simultaneous estimation of metformin and sitagliptin from human plasma was developed and validated. Samples extracted from plasma using acetonitrile were separated on an SCX column and estimated using API 4000 Mass Spectrometer in the positive atmospheric pressure ionization mode (Turboionspray) by following multiple reaction monitoring transitions for both parent and daughter ions. A linear calibration plot was achieved for both the analytes in the concentration ranges of 10-2,206 ng/mL (for metformin) and 3-800.5 ng/mL (for sitagliptin) prepared in K2EDTA pooled plasma. Mean recovery for metformin was 92% and for sitagliptin was 104.5%. It is a fully validated method and successfully applied for estimation of these drug molecules during biostudies.

Cite

CITATION STYLE

APA

Reddy, S., Ahmed, I., Ahmad, I., Mukhopadhyay, A., & Thangam, S. (2015). Development and validation of a method for simultaneous estimation of metformin and sitagliptin in human plasma by LC-MS-MS and its application in a bioequivalence study. Journal of Chromatographic Science, 53(9), 1549–1556. https://doi.org/10.1093/chromsci/bmv055

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free