Integrative description of Macrobiotus kosmali sp. nov. (hufelandi group) from the Island of Madeira (Portugal)

Abstract

Using integrative taxonomy, we describe Macrobiotus kosmali sp. nov. from Ribeira Brava, Madeira (Portugal). Based on morphological and morphometric data from both phase contrast light microscopy (PCM), as well as, from scanning electron microscopy (SEM), description of the new species is provided. Additionally, four DNA markers, three nuclear (18S rRNA, 28S rRNA, ITS-2) and one mitochondrial (COI) were used to calculate the p-distances between Macrobiotus kosmali sp. nov. and other species of the genus Macrobiotus. The uncorrected genetic distances indicated that the new species is the most similar to its four congeners, namely Mac. cf. recens and morphologically, new species is most similar to Mac. dariae, Mac. glebkai, Mac. noemiae, Mac. recens, Mac. rybaki and Mac. scoticus, but differs from them mainly in details of its egg chorion and egg processes. http://urn:lsid:zoobank.org:pub:B371F633-B35C-4578-8C75-9FA2B5E80588.