Gene editing in sorghum through agrobacterium

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Abstract

The application of CRISPR/Cas to introduce targeted genomic edits is powering research and discovery across the genetic frontier. Applying CRISPR/Cas in sorghum can facilitate the study of gene function and unlock our understanding of this robust crop that serves as a staple for some of the most food insecure regions on the planet. When paired with recent advances in sorghum tissue culture and Agrobacteria technology, CRISPR/Cas can be used to introduce desirable changes and natural genetic variations directly into agriculturally relevant sorghum lines facilitating product development. This chapter describes CRISPR/Cas gene editing and provides high-level strategies and expectations for applying this technology using Agrobacterium in sorghum.

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APA

Sander, J. D. (2019). Gene editing in sorghum through agrobacterium. In Methods in Molecular Biology (Vol. 1931, pp. 155–168). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9039-9_11

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