Different sources of acidity in glucose-elicited extracellular acidification in the yeast Saccharomyces cerevisiae

Abstract

Three wild-type strains of Saccharomyces cerevisiae, viz. K, Y55 and Σ1278b, two mutants lacking one or both of the putative K+ transporters, trk1Δ and trk1Δtrk2Δ, and a mutant in the plasma membrane H+-ATPase, viz. pma1-105, were compared in their extracellular acidification following addition of glucose and subsequent addition of KCl; in ATPase activity in purified plasma membranes; and in respiration on glucose. The glucose-induced acidification was the greater the higher the respiratory quotient, i.e. the higher the anaerobic metabolism. A markedly lower acidification was found in the ATPase-deficient pma1-105 strain but also in the TRK-deficient double mutant. The acidification pattern after addition of KCl corresponds to expectations in the TRK mutants; however, a similarly decreased acid production was found in the ATPase-deficient mutant pma1-105. The highest rate of ATP hydrolysis in vitro was found with the trk1Δtrk2Δ mutant where glucose-, as well as KCl-induced acidification were lowest. Likewise, the pma1-105 mutant with extremely low acidification showed only a minutely lower ATP hydrolysis than did its parent Y55 strain. Apparently, several different sources of acidity are involved in the glucose-induced acidification (including extrusion of organic acids); in fact, contrary to the general belief, the H+-ATPase may play a minor role in this process in some strains.