Small intestinal permeability was employed to assess the efficacy of commercially available yoghurts containing probiotics in a rat model of methotrexate (MTX)-induced mucositis. Male Sprague-Dawley rats were allocated to four groups (n = 8): MTX + water, MTX + cow's milk yoghurt (CY; fermented with Lactobacillus johnsonii), MTX + sheep's milk yoghurt (SY; containing Lactobacillus bulgaricus and Streptococcus thermophilus), and saline. Treatment gavage occurred twice daily for 7 days pre-MTX and 5 days post-MTX. Intestinal permeability was assessed on days -7, -1, 2, and 5 of the trial. Intestinal sections were collected at sacrifice for histological and biochemical analyses. Histology revealed that rats receiving CY and SY did not have a significantly damaged duodenum compared to controls. However, an improved small intestinal barrier function was evident, determined by a decreased lactulose/mannitol ratio. Probiotics containing SY and CY may be useful in preventing disruption to intestinal barrier function in MTX-induced mucositis. © 2008 Springer Science+Business Media, LLC.
CITATION STYLE
Southcott, E., Tooley, K. L., Howarth, G. S., Davidson, G. P., & Butler, R. N. (2008). Yoghurts containing probiotics reduce disruption of the small intestinal barrier in methotrexate-treated rats. Digestive Diseases and Sciences, 53(7), 1837–1841. https://doi.org/10.1007/s10620-008-0275-1
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