Pengaruh Durasi Pneumotorak Terhadap Tingkat Stress Oksidatif Paru Tikus Wistar

Abstract

Large pneumothorax with long duration of symptoms can be dangerous. Large pneumothorax can cause hypoxemia and its duration states the duration of hypoxia. This long duration of hypoxia can affect changes in tissue conditions of the lungs, which if it reaches 72 hours can increase the permeability of the alveolar capillaries in the lungs to facilitate pulmonary edema. Large pneumothorax with long duration of symptoms can be a risk factor for re-expansion pulmonary edema (REPE). Meanwhile, hypoxia can also cause increased production of free radicals in lung tissue (pulmonary oxidative stress). This study investigated whether the duration of pneumothorax also affects the level of pulmonary oxidative stress, with experiments in rats. There were 4 groups consisting of 6 rats in each group: 24 hours of pneumothorax (A), 48 hours of pneumothorax (B), 72 hours of pneumothorax (C) and control (D). Pneumothorax is made by injecting air into the right pleural cavity of the chest, then we performed X-ray. All samples were examined for PaO2 to ensure hypoxia status. After that, the rats were examined for malondialdehyde (MDA) levels to express the level of oxidative stress. The result showed that all pneumothorax groups were hypoxemic (PaO2 below 80 mmHg). Mean MDA levels were higher in pneumothorax groups. However, only group C were significantly higher (p=0,031). MDA levels were 1,601 ± 0,739 in group A, 1,585 ± 0,714 in group B, 2,256 ± 0,513 in group C, and 1,243 ± 0,162 in group D. We concluded that pneumothorax can cause pulmonary oxidative stress if the pneumothorax has a large volume and the duration of symptoms reaches 72 hours.