GABAergic signalling in a neurogenic niche of the turtle spinal cord

Abstract

The region that surrounds the central canal (CC) in the turtle spinal cord is a neurogenic niche immersed within already functional circuits, where radial glia expressing brain lipid binding protein (BLBP) behave as progenitors. The behaviour of both progenitors and neuroblasts within adult neurogenic niches must be regulated to maintain the functional stability of the host circuit. In the brain, GABA plays a major role in this kind of regulation but little is known about GABAergic signalling in neurogenic niches of the postnatal spinal cord. Here we explored the action of GABA around the CC of the turtle spinal cord by combining patch-clamp recordings of CC-contacting cells, immunohistochemistry for key components of GABAergic signalling and Ca 2+ imaging. Two potential sources of GABA appeared around the CC: GABAergic terminals and CC-contacting neurones. GABA depolarized BLBP + progenitors via GABA transporter-3 (GAT3) and/or GABA A receptors. In CC-contacting neurones, GABA A receptor activation generated responses ranging from excitation to inhibition. This functional heterogeneity appeared to originate from different ratios of activity of the Na +-K +-2Cl - co-transporter (NKCC1) and the K +-Cl - co-transporter (KCC2). In both progenitors and immature neurones, GABA induced an increase in intracellular Ca 2+ that required extracellular Ca 2+ and was blocked by the selective GABA A receptor antagonist gabazine. Our study shows that GABAergic signalling around the CC shares fundamental properties with those in the embryo and adult neurogenic niches, suggesting that GABA may be part of the mechanisms regulating the production and integration of neurones within operational spinal circuits in the turtle. © 2011 The Authors. Journal compilation © 2011 The Physiological Society.