Phytochemical screening and determination of total phenolic content of Dendophthoe pentandra L. Leaves ethanolic extract on mango host

Abstract

Context: Phytochemical screening and determination of total phenolic content of Loranthus leaves extract at different varieties of mango with a host Manalagi, Arummanis, and Gadung. Aims: To identify the highest total phenolic content of Loranthus leaves extract with different host. Settings and Design: Dendophthoe pentandra L. leaves extract were identified the phytochemical content and determined the total phenolic content. Methods and Material: Using Thin Layer Chromatographic (TLC) test and tube test to identify the chemical substance of D. pentandra leaves extract. Total phenolic contents were determined using spectrophotometry UV-vis method with the Folin-Ciocalteau reagent. Statistical analysis used: ANOVA. Results: TLC test and tube test showed that the extract of D. pentandra contained alkaloids, flavonoids, terpenoids, steroid, and polyphenols, but the tannin compound is found only in the ethanolic extract of Loranthus from Gadung. Total phenolic content in ethanolic extract of D. pentandra leaves with a host Manalagi, Arummanis and Gadung mangoes were respectively 358.03 mg GAE g-1 ± 4.445 mg GAE g-1; 282.869 mg GAE g-1 ± 3.440 mg GAE g-1; and 237.314 mg GAE g-1 ± 4,438 mg GAE g-1. The statistic result of total phenolic content in the sample shows the value of P < 0.05 which means there is a significant difference between total phenolic content in the sample. Conclusions: The highest total phenolic content was D. Pentandra leaves extract from Manalagi mango host.