In mammals and other eukaryotes, most of the genome is transcribed in a developmentally regulated manner to produce large numbers of long noncoding RNAs (lncRNAs). Genome-wide studies have identified thousands of lncRNAs lacking protein-coding capacity. RNA in situ hybridization technique is especially beneficial for the visualization of RNA (mRNA and lncRNA) expression in a heterogeneous population of cells/tissues; however its utility has been hampered by complicated procedures typically developed and optimized for the detection of a specific gene and therefore not amenable to a wide variety of genes and tissues. Recently, bDNA has revolutionized RNA in situ detection with fully optimized, robust assays for the detection of any mRNA and lncRNA targets in formalin-fixed paraffin-embedded (FFPE) and fresh frozen tissue sections using manual processing.
CITATION STYLE
Maqsodi, B., & Nikoloff, C. (2016). Non-isotopic method for In situ LncRNA visualization and quantitation. In Methods in Molecular Biology (Vol. 1402, pp. 165–176). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3378-5_13
Mendeley helps you to discover research relevant for your work.