Changes in the composition of cell wall proteins in barley roots during germination and growth in aluminium presence

Abstract

Root growth inhibition and loss of cell viability in barley root cells were induced by Al during germination of barley caryopses on filter paper moistened with 2mM AlCl3. The inhibition of root growth as well as loss of cell viability started on the third day of germination. This time was also needed for induction of Al-induced changes in the composition of cell wall proteins. The accumulation of three salt-extractable polypeptides with relative molecular mass 14, 27, and 29 kDa started 72 h after the beginning of germination of barley caryopses on Al containing filter paper. However, the inhibition of developmentally regulated deposition of three (18, 23 and 28 kDa) salt-extractable CW polypeptides was observed at the same time. The pattern of detergent-extractable CW proteins was not influenced by Al regardless of the duration of Al treatment.