Cell-based microarrays using superhydrophobic platforms patterned with wettable regions

Abstract

The use of patterned platforms to print cellular arrays enables the high-throughput study of cell behavior under a multitude of different conditions. This rapid, cost-saving and systematic way of acquiring biologically relevant information has found application in diverse scientific and industrial fields. In an initial stage of development, platforms targeting high-throughput cellular studies were restricted to standard two-dimensional (2D) setups. The design of novel platforms compatible with three-dimensional (3D) cell culture arose after the elucidation of the extreme importance of culturing cells in matrices resembling the native extracellular matrix–cells and cell–cell interactions. This need for biomimetic environments has been established in fields like drug discovery and testing, disease model development, and regenerative medicine. Here, we provide a description of the processing of flat platforms based on wettability contrast, compatible with the high-throughput generation and study of cell response in 3D biomaterials, including cell-laden hydrogels and porous 3D scaffolds. The application of the aforementioned platforms to produce 3D microtissues, which may find application as tissue models for drug screening or as biomimetic building blocks for tissue engineering, is also addressed. In this chapter, a description of the steps for (1) high-throughput platform processing, (2) deposition of cell and biomaterial arrays, and (3) image-based results screening is provided.