Electron microscopy of mammalian chromosomes.

Abstract

This chapter describes methods of preparation of meiotic nuclei for their analysis with electron microscopy. Procedures include surface spreading as well as fixation, staining, and sectioning of tissue and subsequent 3-D reconstruction analysis. Immunostaining protocols for electron microscopy are provided. The rationale for using electron microscopy in place of light or fluorescent microscopy for specific cytological analyses is presented.