Resistance validation of several chili peppers to anthracnose (Colletrothicum acutatum) isolate Sukabumi

Abstract

Anthracnose Colletrothicum acutatum is the most dominant and virulent disease that attacks hot pepper pants and can reduce yields. One of the ways to control anthracnose can use resistant varieties. The research objective was to ensure the resistance of the hot pepper lines to C. acutatum isolate from Sukabumi. Anthracnose isolates used C. acutatum from Sukabumi with a DNA band measuring 490 bp. The method used to test the resistance to anthracnose on hot pepper was by inoculating 5 ul of conidial suspension with a density of 5.0 × 105 as much as one injection per fruit. PR protein analysis was performed on anthracnose infected and healthy fruit. The results showed that: (1). Based on the morphology of the isolates, the top looks are white and gray, and beige, white, peach for the colony color is shown below. (2). C. acutatum isolates from Sukabumi were identified by PCR method with DNA bands measuring 490 bp. (3). The results of validation of resistance to anthracnose C. acutatum with Sukabumi isolates obtained resistant lines: 2A (R-4), 4A (R-15), 4B (R-9), 6 (R-12), 7 (-13), 8 (R-14) with a lesion diameter less than 5 mm. (4). The absorbance value of peroxidase enzyme and protein content of anthracnose-resistant fruit was higher than that of susceptible fruit. (5). The results of the analysis of the protein band pattern profile on the PR-protein results showed that in certain molecular weight range thin and thick gene expressions were seen with molecular weights ranging between 37 kDa.