Perakine reductase (PR) catalyzes an NADPH-dependent step in a side-branch of the 10-step biosynthetic pathway of the alkaloid ajmaline. The enzyme was cloned by a "reverse-genetic" approach from cell suspension cultures of the plant Rauvolfia serpentina (Apocynaceae) and functionally expressed in Escherichia coli as the N-terminal His6-tagged protein. PR displays a broad substrate acceptance, converting 16 out of 28 tested compounds with reducible carbonyl function which belong to three substrate groups: benzaldehyde, cinnamic aldehyde derivatives and monoterpenoid indole alkaloids. The enzyme has an extraordinary selectivity in the group of alkaloids. Sequence alignments define PR as a new member of the aldo-keto reductase (AKR) super family, exhibiting the conserved catalytic tetrad Asp52, Tyr57, Lys84, His126. Site-directed mutagenesis of each of these functional residues to an alanine residue results in >97.8% loss of enzyme activity, in compounds of each substrate group. PR represents the first example of the large AKR-family which is involved in the biosynthesis of plant monoterpenoid indole alkaloids. In addition to a new esterase, PR significantly extends the Rauvolfia alkaloid network to the novel group of peraksine alkaloids. © 2008 Springer Science+Business Media B.V.
CITATION STYLE
Sun, L., Ruppert, M., Sheludko, Y., Warzecha, H., Zhao, Y., & Stöckigt, J. (2008). Purification, cloning, functional expression and characterization of perakine reductase: The first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Molecular Biology, 67(5), 455–467. https://doi.org/10.1007/s11103-008-9331-7
Mendeley helps you to discover research relevant for your work.