Instability of repetitive DNA sequences causes numerous hereditary disorders in humans, the majority of which are associated with trinucleotide repeat expansions. Here, we describe a unique system to study instability of triplet repeats in a yeast experimental setting. Using fluctuation assay and the novel program FluCalc we are able to accurately estimate the rates of large-scale expansions, as well as repeat-mediated mutagenesis and gross chromosomal rearrangements for different repeat sequences.
CITATION STYLE
Radchenko, E. A., McGinty, R. J., Aksenova, A. Y., Neil, A. J., & Mirkin, S. M. (2018). Quantitative analysis of the rates for repeat-mediated genome instability in a yeast experimental system. In Methods in Molecular Biology (Vol. 1672, pp. 421–438). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7306-4_29
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