Application of NMR for quantification of magnetic nanoparticles and development of paper-based assay

Abstract

H1 NMR relaxometry is a method that is extremely sensitive to the presence of magnetic nanoparticles, which significantly affect the transverse relaxation time of the water proton. Accordingly, the use of magnetic nanoparticles as labels allows detection of even extremely small amounts of the test substance. This paper analyzes the prospects for applying the method of solid-phase NMR-relaxometric determination of biologically active molecules. The nitrocellulose membranes are chosen as a solid phase and nanoparticles based on iron core with a carbon shell are used as magnetic labels. The possibility of detecting small concentrations of magnetic particles in porous medium is demonstrated. Finally, the ability to detect extremely low concentrations of an analyte, in this case, streptavidin protein (0.5 ng/ml to 100 ng/ml), which is actively used in various fields of biology and medicine, is demonstrated.