Tips and techniques for processing and sectioning undecalcified murine bone specimens

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Abstract

Preparation of mineralized tissue specimens for bone-specific staining encompasses a critical sequence of histological techniques that provides visualization of tissue and cellular morphology. Bone specimens are fixed in 10 % neutral-buffered formalin, dehydrated in graded ethanol (EtOH) solutions (and optionally cleared in xylene), infiltrated and embedded in polymethyl methacrylate (methyl methacrylate), classically sliced into 4-10 micrometer (μm) sections, and stained with bone-specific histological stains such as von Kossa (with either nuclear fast red solution counterstain or MacNeal's tetrachrome counterstain), modified Goldner's trichrome, and alizarin red S stain. Here, we describe the tissue processing of mineralized mouse bones from dissection to staining for histological analysis by light microscopy. © 2014 Springer Science+Business Media, LLC.

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Bemenderfer, T. B., Harris, J. S., Condon, K. W., & Kacena, M. A. (2014). Tips and techniques for processing and sectioning undecalcified murine bone specimens. Methods in Molecular Biology, 1130, 123–147. https://doi.org/10.1007/978-1-62703-989-5_10

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