Inhibitory effect of estradiol-17β and progesterone on bactericidal activity in uteri of rabbits infected with Escherichia coli

Abstract

The influence of ovarian hormones at different estrous stages on the bactericidal activity of the uterus in rabbits was investigated. When Escherichia coli cells were inoculated in ligated uteri, the survival period of the bacteria in the uterus at the luteal phase was clearly longer than that at the follicular phase. At the luteal phase, high levels of plasma estradiol-17β and progesterone were detected. A luteolytic treatment with prostaglandin F(2α) and human chorionic gonadotropin at the luteal phase lowered plasma progesterone levels and prompted bacterial clearance form the uterus. In ovariectomized rabbits, E. coli from the uterine exudates was not detected 6 days after the inoculation in both the nontreated and estradiol-17β - treated animals. In the progesterone - treated rabbits, the survival period of E. coli was longer than that in the nontreated and estradiol-17β - treated animals. When estradiol-17β and progesterone at the ratio of 1:100 were administrated concurrently, E. coli survived for the longest period in the rabbits treated with various doses of different hormones. Formalin-killed E. coli cells were inoculated into the uterine lumen, and 4 h later the proportion of heterophils phagocytizing the bacteria dropped in the progesterone - treated rabbits and in the estradiol-17β - and progesterone - treated rabbits, but there was no significant difference in heterophil numbers among the rabbits treated with different hormones. The present results suggest that progesterone inhibits the bactericidal activity of the uterus and that estrogen concurrently secreted at the luteal phase promotes the inhibitory action of progesterone, althouhg estrogen alone hardly affects the uterine defense. In addition, the lowering of the bactericidal activity of the uterus at the luteal phase may be attributable to lower activity of phagocytosis by heterophils infiltrated into the uterine lumen.