Physiological and growth responses of C3 and C4 plants to reduced temperature when grown at low CO2 of the last ice age

Abstract

During the last ice age, CO2 concentration ([CO2]) was 180-200 μmol/mol compared with the modern value of 380 μmol/mol, and global temperatures were ∼8°C cooler. Relatively little is known about the responses of C 3 and C 4 species to long-term exposure to glacial conditions. Here Abutilon theophrasti Medik. (C3) and Amaranthus retroflexus L. (C4) were grown at 200 μmol/mol CO2 with current (30/24 °C) and glacial (22/16 °C) temperatures for 22d. Overall, the C4 species exhibited a large growth advantage over the C3 species at low [CO2]. However, this advantage was reduced at low temperature, where the C4 species produced 5 × the total mass of the C3 species versus 14 × at the high temperature. This difference was due to a reduction in C4 growth at low temperature, since the C3 species exhibited similar growth between temperatures. Physiological differences between temperatures were not detected for either species, although photorespiration/net photosynthesis was reduced in the C3 species grown at low temperature, suggesting evidence of improved carbon balance at this treatment. This system suggests that C4 species had a growth advantage over C3 species during low [CO2]of the last ice age, although concurrent reductions in temperatures may have reduced this advantage. © 2008 Institute of Botany, the Chinese Academy of Sciences.