Host prophenoloxidase expression in freshwater crayfish is linked to increased resistance to the crayfish plague fungus, Aphanomyces astaci

Abstract

The crayfish plague (Aphanomyces astaci) susceptible freshwater crayfish Astacus astacus and the resistant species Pacifastacus leniusculus were compared with respect to differential haemocyte count and expression of prophenoloxidase and peroxinectin. A major difference found was that resistant crayfish continuously produced high levels of prophenoloxidase (proPO) transcripts and that these levels could not be further increased, whereas in susceptible crayfish proPO transcript levels and resistance were augmented by immunostimulants. In As. astacus this could be registered as higher proPO transcript levels in the semigranular population of haemocytes and to an increased survival time after experimental infections with A. astaci.