Competition assays using barcoded deletion strains to gain insight into small RNA function

Abstract

Ordered collections of barcoded deletion mutants can be screened rapidly in mixed cultures to uncover resistance and sensitivity phenotypes associated with the loss of a gene. As such, they are invaluable tools for assigning gene function in the post-genomic era. In this protocol, we describe methodologies for creating and employing barcoded deletion mutants in competitive screens as well as how to analyze the ensuing results. © 2012 Springer Science+Business Media, LLC.