Cell migration analysis of cortical interneurons after electroporation

Abstract

Since developmental processes of the nervous system such as neuronal migration are dynamic ones, observation of living neurons in the developing brain provides important insights into understanding the mechanisms of neuronal development. In this chapter, I describe methods for visualizing a specific subset of neurons, cortical interneurons, and precerebellar neurons in vitro or in living mouse embryos as well as those for the analysis of the dynamics of their migratory behaviors.