Improved sodium hydroxide digestion method without homogenization for extraction of gentamicin from renal tissue

Abstract

Recovery of gentamicin from renal cortical tissue (assayed by fluorescence polarization immunoassay) was highest (P < 0.05) when using a sodium hydroxide (NaOH) digestion procedure without homogenization (90.0 ± 5.4%), followed by homogenization with NaOH digestion (85.8 ± 7.7%) and homogenization with trichloroacetic acid precipitation (84.4 ± 3.3%). Simple homogenization recovered the least gentamicin (59.0 ± 5.2%; P < 0.05).