BackgroundAcute kidney injury (AKI) in humans has few therapeutic options. In experimental models, administration of progenitor cells facilitates recovery from AKI. Human umbilical cord-derived CD133+ progenitor cells promote endothelial repair in ischemic limb, heart and brain tissue.MethodsWe examined the effects of human CD133+ progenitor cells in bilateral ischemia-reperfusion (I/R) kidney injury in non-obese diabetic severe combined immunodeficient mice. CD133+ cells from human cord blood were injected intravenously at the time of reperfusion and the extent of injury was determined by plasma biochemistry and kidney histology.ResultsIn mice with I/R, fluorescently labeled CD133+ cells were detected in blood 2 min after injection but decreased rapidly thereafter with no evidence of homing to the kidneys. In mice subjected to I/R, CD133+ cells significantly increased plasma urea and Cr at 24 h compared to vehicle-or CD133- cell-treated mice. CD133+ cells exacerbated tubular necrosis and apoptosis, increased plasma tumor necrosis factor-α and increased kidney neutrophil infiltration. In contrast, CD133+ cells did not affect tubular cell proliferation. Administration of CD133+ cells to FVB/N mice post-I/R also augmented kidney injury.ConclusionsThese data indicate that human cord blood-derived CD133+ cells unexpectedly exacerbate ischemic AKI in mice, possibly through soluble factors. Our study highlights the importance of caution in cell-based therapies for human AKI. © 2012 The Author.
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Burger, D., Gutsol, A., Carter, A., Allan, D. S., Touyz, R. M., & Burns, K. D. (2012). Human cord blood CD133+ cells exacerbate ischemic acute kidney injury in mice. Nephrology Dialysis Transplantation, 27(10), 3781–3789. https://doi.org/10.1093/ndt/gfs110