The FTD ‐like syndrome causing TREM 2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism

Abstract

Genetic variants in the triggering receptor expressed on myeloid cells 2 ( TREM 2) increase the risk for several neurodegenerative diseases including Alzheimer's disease and frontotemporal dementia ( FTD ). Homozygous TREM 2 missense mutations, such as p.T66M, lead to the FTD ‐like syndrome, but how they cause pathology is unknown. Using CRISPR /Cas9 genome editing, we generated a knock‐in mouse model for the disease‐associated Trem2 p.T66M mutation. Consistent with a loss‐of‐function mutation, we observe an intracellular accumulation of immature mutant Trem2 and reduced generation of soluble Trem2 similar to patients with the homozygous p.T66M mutation. Trem2 p.T66M knock‐in mice show delayed resolution of inflammation upon in vivo lipopolysaccharide stimulation and cultured macrophages display significantly reduced phagocytic activity. Immunohistochemistry together with in vivo TSPO small animal positron emission tomography (μ PET ) demonstrates an age‐dependent reduction in microglial activity. Surprisingly, perfusion magnetic resonance imaging and FDG ‐μ PET imaging reveal a significant reduction in cerebral blood flow and brain glucose metabolism. Thus, we demonstrate that a TREM 2 loss‐of‐function mutation causes brain‐wide metabolic alterations pointing toward a possible function of microglia in regulating brain glucose metabolism. image Genetic variants in the triggering receptor expressed on myeloid cells 2 ( TREM 2) increase the risk for several neurodegenerative diseases including Alzheimer's disease and frontotemporal dementia ( FTD ). Homozygous endogenous expression of the TREM 2 p.T66M mutation impairs microglia function, brain perfusion and glucose metabolism, suggesting that microglial TREM 2 acts as a signaling hub. Trem2 p.T66M knock‐in mice show delayed resolution of inflammation. Trem2 p.T66M knock‐in mice exhibit decreased age‐dependent microglial activity. Trem2 p.T66M knock‐in mice show reduced cerebral blood flow. Trem2 p.T66M knock‐in mice show reduced glucose metabolism.