Immobilization of chondroitin sulfate a onto monolithic epoxy silica column as a new chiral stationary phase for high-performance liquid chromatographic enantioseparation

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Abstract

Chondroitin sulfate A was covalently immobilized onto a monolithic silica epoxy column involving a Schiff base formation in the presence of ethylenediamine as a spacer and evaluated in terms of its selectivity in enantioseparation. The obtained column was utilized as a chiral stationary phase in enantioseparation of amlodipine and verapamil using a mobile phase consisting of 50 mM phosphate buffer pH 3.5 and UV detection. Sample dilution by organic solvents (preferably 25% v/v acetonitrile-aqueous solution) was applied to achieve baseline enantioresolution (Rs > 3.0) of the individual drug models within 7 min, an excellent linearity (R2 = 0.999) and an interday repeatability of 1.1% to 1.8% RSD. The performance of the immobilized column for quantification of racemate in commercial tablets showed a recovery of 86–98% from tablet matrices. Computational modeling by molecular docking was employed to investigate the feasible complexes between enantiomers and the chiral selector.

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APA

Ratih, R., Wätzig, H., Azminah, A., Asmari, M., Peters, B., & Deeb, S. E. (2021). Immobilization of chondroitin sulfate a onto monolithic epoxy silica column as a new chiral stationary phase for high-performance liquid chromatographic enantioseparation. Pharmaceuticals, 14(2), 1–14. https://doi.org/10.3390/ph14020098

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