ATP depletion affects NPM translocation and exportation of rRNA from nuclei

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Abstract

Nucleophosmin/B23 (NPM) is a nucleolar phosphoprotein which shifts from nucleoli to the nucleoplasm in cells treated with certain cytotoxic agents (NPM-translocation). NPM requires GTP for localization into nucleoli. To understand more about NPM's dynamic localization, the effects of lowering ATP on NPM-translocation and rRNA synthesis were studied. When the ATP level in HeLa cells was reduced by sodium azide, NPM-translocation was blocked. Similar results were obtained when ATP was depleted by other agents, suggesting that ATP depletion was responsible for the blocking of NPM-translocation. It was found that newly synthesized rRNA accumulated in the nuclei during ATP-depletion. Significantly larger than normal nucleoli were also observed. These results indicate that NPM may be involved in the transportation of newly synthesized ribosomes.

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Finch, R. A., & Chan, P. K. (1996). ATP depletion affects NPM translocation and exportation of rRNA from nuclei. Biochemical and Biophysical Research Communications, 222(2), 553–558. https://doi.org/10.1006/bbrc.1996.0782

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