Evaluation of the polymerase chain reaction method for identification of Vibrio vulnificus isolated from marine environments

31Citations
Citations of this article
12Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

The polymerase chain reaction (PCR) method for identification of Vibrio vulnificus in the marine environment was evaluated by comparing it to both the conventional and DNA-DNA hybridization methods. Of 13,325 isolates obtained from seawater and sediment samples, and oyster and goby specimens collected from the coastal waters of Tokyo Bay, Japan, only 61 isolates were identified as V. vulnificus on the basis of phenotypic characteristics and the amplification of the cytotoxin-hemolysin gene by the PCR method. All 61 isolates were further confirmed to be V. vulnificus by a DNA-DNA hybridization method and the API 20E system although they were divided into 13 groups on the basis of their API 20E profiles. These results strongly suggest that the PCR method is useful for identification of this organism.

Cite

CITATION STYLE

APA

Aono, E., Sugita, H., Kawasaki, J., Sakakibara, H., Takahashi, T., Endo, K., & Deguchi, Y. (1997). Evaluation of the polymerase chain reaction method for identification of Vibrio vulnificus isolated from marine environments. Journal of Food Protection, 60(1), 81–83. https://doi.org/10.4315/0362-028X-60.1.81

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free