Active DNA demethylation plays important roles in the epigenetic reprogramming of developmental processes. 5-formylcytosine (5fC) is produced during active demethylation of 5-methylcytosine (5mC). Here, we describe a technique called CLEVER-seq (Chemical-labeling-enabled C-to-T conversion sequencing), which detects the whole genome 5fC distribution at single-base and single-cell resolution. CLEVER-seq is suitable for the analysis of precious samples such as early embryos and laser microdissection captured samples.
CITATION STYLE
Zhu, C., Gao, Y., Peng, J., Tang, F., & Yi, C. (2019). Single-cell 5fC sequencing. In Methods in Molecular Biology (Vol. 1979, pp. 251–267). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9240-9_16
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