In vitro myristoylation assay of arabidopsis proteins

3Citations
Citations of this article
7Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Myristoylation is a lipid modification conserved among eukaryotes and involves the addition of a 14-carbon myristoyl moiety to a glycine at the N-terminus of cargo proteins. Since not every protein with an N-terminal glycine is myristoylated, experimental verification is necessary to determine which proteins are indeed myristoylated. Here we describe an in vitro myristoylation assay for the Arabidopsis heterotrimeric G protein alpha subunit, GPA1, as well as the Arabidopsis SALT OVERLY SENSITIVE3. This method can be easily adopted to other proteins of interest. © Springer Science+Business Media, LLC 2013.

Cite

CITATION STYLE

APA

Feng, X., Shi, W., Wang, X., & Running, M. P. (2013). In vitro myristoylation assay of arabidopsis proteins. Methods in Molecular Biology, 1043, 135–139. https://doi.org/10.1007/978-1-62703-532-3_14

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free