Imaging intracellular Ca2+ in cardiomyocytes with genetically encoded fluorescent probes

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Abstract

Calcium (Ca2+) is a key player in cardiomyocyte homeostasis, and its roles span from excitation-contraction coupling to metabolic and structural signaling. Alterations in the function or expression of Ca2+-handling proteins are common findings in failing cardiomyocytes, which have been linked to impaired contractility and detrimental remodeling of the cellular structure. For these reasons, the study of intracellular Ca2+ handling in cardiomyocytes represents a central method in experimental molecular cardiology.

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Campo, A., & Mongillo, M. (2019). Imaging intracellular Ca2+ in cardiomyocytes with genetically encoded fluorescent probes. In Methods in Molecular Biology (Vol. 1925, pp. 111–125). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9018-4_10

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