G-protein-coupled receptors (GPCRs) play a major role in psychiatric disorders and are the targets of several current therapeutic approaches in this field. A number of studies have now shown that GPCRs can assemble as high molecular weight homo- and hetero-oligomers, which could affect ligand binding, intracellular signalling or trafficking. This information could be critical in design of new drugs to treat neurological and psychiatric disorders. This chapter describes a sequential co-immunoprecipitation and immunoblot protocol for determining oligomerisation of the 5-hydroxytryptamine (HT)1A receptor with other GPCRs in co-transfected HEK-293 cells.
CITATION STYLE
Guest, P. C. (2017). Sequential co-immunoprecipitation and immunoblot approach to determine oligomerisation of g-protein-coupled receptors. In Advances in Experimental Medicine and Biology (Vol. 974, pp. 237–243). Springer New York LLC. https://doi.org/10.1007/978-3-319-52479-5_20
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