The use of stable-isotope probing (SIP) allows tracing specific labeled substrates into fungi leading to a better understanding of their role in biogeochemical cycles and their relationship with their environment. Stable isotope probing combined with ribosomal RNA molecule, conserved in the three kingdoms of life, and messenger RNA analysis permits the linkage of diversity and function. Here, we describe two methods designed to investigate the interactions between plants and their associated mycorrhizal compartment by tracing carbon flux from the host plant to its symbionts.
CITATION STYLE
Van, A. L., Duhamel, M., Quaiser, A., & Vandenkoornhuyse, P. (2023). Stable Isotope Probing-RNA Strategy to Study Plant/Fungus Interactions. In Methods in Molecular Biology (Vol. 2605, pp. 169–186). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2871-3_9
Mendeley helps you to discover research relevant for your work.