DNA Damage Caused by Photoexcited Ketoprofen Measured by the Comet Assay

Abstract

Using the Comet assay, the extent of DNA damage from the interaction of Ketoprofen with UV A/UVB and DNA was evaluated. Ketoprofen was injected into the isolated monomol. white blood cell culture, and the cells were then incubated and subsequently mixed with agarose and casted onto a thin film. At 0.1 MED, an increase in DNA damage was noted only when Ketoprofen was present at 10-5 M. Ketoprofen at 10-6 M and 10-4 M showed a little more or the same amt. of DNA damage, resp. At 0.2 MED, Ketoprofen at 10-6 M displayed the same amt. of DNA damage as the control, while Ketoprofen at 10-5 and 10-4M decreased in DNA damage compared to control. These findings demonstrated that Ketoprofen has a protective effect on the DNA. When Ketoprofen is present, it filters the UV light, interfering with the DNA absorption of the radiation, preventing it from being damaged. [on SciFinder (R)]