Cystic fibrosis transmembrane conductance regulator (CFTR) anion channel, a member of ABC transporter superfamily, gates following ATP-dependent conformational changes of the nucleotide binding domains (NBD). Reflecting the hundreds of milliseconds duration of the channel open state corresponding to the dimerization of two NBDs, macroscopic WT-CFTR currents usually showed a fast, single exponential relaxation upon removal of cytoplasmic ATP. Mutations of tyrosine1219, a residue critical for ATP binding in second NBD (NBD2), induced a significant slow phase in the current relaxation, suggesting that weakening ATP binding affinity at NBD2 increases the probability of the stable open state. The slow phase was effectively diminished by a higher affinity ATP analogue. These data suggest that a stable binding of ATP to NBD2 is required for normal CFTR gating cycle, andthat the instability of ATP binding frequently halts the gating cycle in the open state presumably through a failure of ATP hydrolysis at NBD2. © 2010 The Physiological Society of Japan and Springer.
CITATION STYLE
Shimizu, H., Yu, Y. C., Kono, K., Kubota, T., Yasui, M., Li, M., … Sohma, Y. (2010). A stable ATP binding to the nucleotide binding domain is important for reliable gating cycle in an ABC transporter CFTR. Journal of Physiological Sciences, 60(5), 353–362. https://doi.org/10.1007/s12576-010-0102-2
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