Polymeric multilayered capsules (PMCs) have found great applicability in bioencapsulation, an evolving branch of tissue engineering and regenerative medicine. Here, we describe the production of hierarchical PMCs composed by an external multilayered membrane by layer-by-layer assembly of poly(L-lysine), alginate, and chitosan. The core of the PMCs is liquified and encapsulates human adipose stem cells and surface-functionalized collagen II-TGF-β3 poly(L-lactic acid) microparticles for cartilage tissue engineering
CITATION STYLE
Correia, C. R., Reis, R. L., & Mano, J. F. (2015). Nanostructured capsules for cartilage tissue engineering. In Methods in Molecular Biology (Vol. 1340, pp. 181–189). Humana Press Inc. https://doi.org/10.1007/978-1-4939-2938-2_13
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