Pigment epithelium-derived factor receptor (PEDF-R) is a patatin-like phospholipase domain-containing 2 (PNPLA2) protein recently identified in the retina. It has high affinity for pigment epithelium-derived factor (PEDF), a protein that acts on the neural and vascular retina. PEDF-R is a membrane-linked phospholipase and PEDF binding stimulates its enzymatic phospholipase A 2 activity, which catalyzes the cleavage of fatty acids from membrane phospholipids. In this study, we mapped the epitope of an antibody for PEDF-R using recombinant PEDF-R polypeptides fragments and synthetic peptides. In addition to mapping the recognition site of the antibody on PEDF-R, we have identified peptides that specifically block its immunoreactivity and will prove to be useful tools for PEDF-R research. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Subramanian, P., Rapp, M., & Becerra, S. P. (2012). Identification of Pigment Epithelium-Derived Factor Receptor (PEDF-R) antibody epitopes. In Advances in Experimental Medicine and Biology (Vol. 723, pp. 799–805). https://doi.org/10.1007/978-1-4614-0631-0_102
Mendeley helps you to discover research relevant for your work.