Background. Parasite concentration methods facilitate molecular, biochemical and immunological research on the erythrocytic stages of Plasmodium. In this paper, an adaptation of magnetic MACS® columns for the purification of human Plasmodium species is presented. This method was useful for the concentration/purification of either schizonts or gametocytes. Results and conclusions. The magnetic removal of non-parasitized red blood cells (in vivo and in vitro) using magnetic columns (MACS) was evaluated. This easy-to-use technique enriched schizonts and gametocytes from Plasmodium falciparum in vitro cultures with a very high degree of purity. In addition, all haemozoin-containing stages (schizonts and/or gametocytes) from the peripheral blood of infected patients could be concentrated using this method. This method is particularly useful for the concentration of non-falciparum species, which do not grow in culture and are otherwise difficult to obtain in large amounts. © 2008 Ribaut et al; licensee BioMed Central Ltd.
CITATION STYLE
Ribaut, C., Berry, A., Chevalley, S., Reybier, K., Morlais, I., Parzy, D., … Valentin, A. (2008). Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species. Malaria Journal, 7. https://doi.org/10.1186/1475-2875-7-45
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