Inter simple sequence repeat (ISSR) markers and pedigree information to assess genetic diversity and relatedness within raspberry genotypes

Abstract

Genetic diversity and relatedness among nine North American red raspberry (Rubus idaeus L.) cultivars and four Canadian breeding lines were studied using inter simple sequence repeat (ISSR) markers and pedigree analysis. Eighteen primers generated 306 polymorphic ISSR-PCR bands. Cluster analysis by the unweighted pair-group method with arithmetic averages (UPGMA) revealed a substantial degree of genetic diversity among the 13 genotypes, similarity values ranged from 24% to 49% that were in agreement with the principal coordinate (PCO) analysis. Geographical distribution for the place of breeding program explained only 1.0% of total variation as revealed by analysis of molecular variance (AMOVA). The UPGMA clustering for coancestry identified 3% to 25% similarity among the nine cultivars. The correlation coefficient between the genetic similarity values calculated from the Jaccard-based ISSR data and pairwise coefficients of coancestry of nine cultivars was positive but insignificant. The ISSR markers detected a sufficient degree of polymorphism to differentiate among raspberry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in the current raspberry breeding program. © 2007 by The Haworth Press.