Attachment of bacteria to the roots of higher plants

Abstract

Attachment of soil bacteria to plant cells is supposedly the very early step required in plant-microbe interactions. Attachment also is an initial step for the formation of microbial biofilms on plant roots. For the rhizobia-legume symbiosis, various mechanisms and diverse surface molecules of both partners have been proposed to mediate in this process. The first phase of attachment is a weak, reversible, and unspecific binding in which plant lectins, a Ca +2-binding bacterial protein (rhicadhesin), and bacterial surface polysaccharide appear to be involved. The second attachment step requires the synthesis of bacterial cellulose fibrils that cause a tight and irreversible binding of the bacteria to the roots. Cyclic glucans, capsular polysaccharide, and cellulose fibrils also appear to be involved in the attachment of Agrobacterium to plant cells. Attachment of Azospirillum brasilense to cereals roots also can be divided in two different steps. Bacterial surface proteins, capsular polysaccharide and flagella appear to govern the first binding step while extracellular polysaccharide is involved in the second step. Outer cell surface proteins and pili are implicated in the adherence of Pseudomonas species to plant roots. © 2007 Federation of European Microbiological Societies.