In this article, we provide a method to isolate primary epidermal melanocytes from reporter mice, which also allow targeted gene inactivation. The mice from which these cells are isolated are bred into a Rosa26mT/mG reporter background, which results in GFP expression in the targeted melanocytic cell population. These cells are isolated and cultured to >95% purity. The cells can be used for gene expression studies, clonogenic experiments, and biological assays, such as capacity for migration. Melanocytes are slow moving cells, and we also provide a method to measure motility using individual cell tracking and data analysis.
CITATION STYLE
Dagnino, L., & Crawford, M. (2019). Isolation, culture, and motility measurements of epidermal melanocytes from GFP-expressing reporter mice. In Methods in Molecular Biology (Vol. 1879, pp. 243–256). Humana Press Inc. https://doi.org/10.1007/7651_2018_116
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